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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Posttranscriptional RNA stabilization of telomeric RNAs FRG2, DBE-T, D4Z4 at human 4q35 in response to genotoxic stress and D4Z4 macrosatellite repeat length

Fig. 5

4q35 genes regulation upon different stimuli reflects architectural and epigenetic patterns. A, B RNA fractionation experiments were conducted in CTRL (A) (n = 2) and DRA:4U (n = 2) (B) HPMs. Transcripts from the indicated 4q35 genes were measured by qPCR analysis of cytoplasmic, nuclear and chromatin-associated RNA fractions, and the percentage detected in each fraction over total RNA was graphed. GAPDH, TERRA and NEAT1 transcripts were also assessed as positive controls that are most enriched in cytoplasmic, chromatin and nuclear fractions, respectively. C Chromatin-RNA Immunoprecipitation (ChRIP) experiment performed in HPMs. Antibodies directed to H3K4me3, H3K9me3, H3K27me3 were used to precipitate RNA from CTRL and DRA:4U cells. Data shown are means ± s.e.m. of three replicates. * (asterisks) refer to each different antibody used in ChRIP experiment to show the statistical significance of data obtained for each antibody over control IgG

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Clinical Epigenetics

ISSN: 1868-7083

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